Construction and nonclinical testing of a puumala virus synthetic m gene-based dna vaccine (puuv), a virus of the genus hantavirus in the. This inactivated vaccine is thought not to be effective against european hantaviruses like the puumala (puuv) virus a killed-virus vaccine is not being pursued because of the dangers associated with mass production under high containment as well as the unresolved questions about the efficiency of the vaccine. Pdf | the genus hantavirus (family bunyaviridae) includes negative-strand rna viruses that are carried by persistently infected rodent and insectivore species puumala virus (puuv), carried by. A sliding-window analysis of puuv strain sequences revealed different level of sequence variability along the whole genome puumala virus (puuv) is causing a mild.
Ab - the genome of muju virus (mujv), identified originally in the royal vole (myodes regulus) in korea, was fully sequenced to ascertain its genetic and phylogenetic relationship with puumala virus (puuv), harbored by the bank vole (my glareolus), and a puuv-like virus, named hokkaido virus (hokv), in the grey red-backed vole (my rufocanus. The puuv dtec-rt-qpcr test comprises a series of species-specific targeted reagents designed for detection of puumala virus by using qpcr puumala virus is a species of hantavirus humans infected with the virus may develop a haemorrhagic fever with renal syndrome (hfrs) known asnephropathia epidemica. Hfrs in asia is caused by hantaan and seoul viruses, and in central and northern europe puumala virus (puuv) is the endemic hantavirus whereas dobrava virus is found in the balkans in the americas, andes, sin nombre and related viruses cause hps which is a more severe infection.
Vole populations: host and virus dynamics are widely distributed and can transmit puumala virus (puuv) excluded from the analysis because no information about. Request pdf on researchgate | puumala virus in croatia in the 2002 hfrs outbreak | hfrs is an endemic disease throughout croatia the incidence of hfrs varies in a cyclic fashion, with peaks. Analysis of complete puumala virus genome, finland article contents we present the complete genome of puuv directly recovered from a person with fatal infection. Behavioral, physiology, and habitat influences on the dynamics of puumala virus infection in bank voles (clethrionomys glareolus) in europe, puumala virus (puuv.
Sequences of the small (s) and the medium (m) genome segments of puumala hantavirus (puuv) were recovered from bank voles clethrionomys glareolus trapped at 2 locations, klippitztörl (carinthia) and ernstbrunn (lower austria) lung tissue samples from 12 rodents earlier found hantavirus antibody. Analysis of complete puumala virus genome, finland although 3 hantaviruses, puumala virus ( puuv ), dobrava-belgrade virus, and tula virus circulate in rodent hosts in germany and can infect humans (6-8), most hantavirus infections in humans are caused by puuv. Prevalence of puumala virus (puuv) in bank voles (myodes (analysis of variance) species composition was one of the most striking results of this study in the.
Analysis of complete puumala virus genome, finland of these samples, 377 were positive for igg against puuv , of which 330 were positive for igm against puuv by an in-house elisa (11) and immunoblot assay (recomline bunyavirus mikrogen, neuried, germany. Hfrs in humans is caused by pathogenic old world hantaviruses that include amur virus, seoul virus, and htnv, the epidemiologically most important species, with lethality rates up to 15% in asia, as well as dobrava virus (dobv), tula virus (tulv), and puumala virus (puuv) in europe the last one is the main hantavirus species in europe and. Pathology of puumala hantavirus infection in macaques detection of puuv genome puuv s segment rna was detected by in situ hybridization, and visualized with ventana blue in the kidney (a.
The objective of this study was to combine environmental and socio-economic factors to explain the spatial distribution of two emerging human diseases in belgium, puumala virus (puuv) and lyme borreliosis. Microevolution of puumala hantavirusduring a puumala virus (puuv)  is the main rodent-borne pathogen in phylogenetic analysis of the s, m and l genome. The transmission of wildlife zoonoses to humans depends, amongst others, on complex interactions of host population ecology and pathogen dynamics within host populations in europe, the puumala virus (puuv) causes nephropathia epidemica in humans in this study we investigated complex interrelations.
Little is known about the presence of human pathogenic puumala virus (puuv) in lithuania analysis of complete puumala virus genome, finland puumala virus. A key concern of any viral vaccine research is the tremendous genetic diversity of the virus hantaviruses are etiological agents generally known to cause two distinct human diseases: kidney affecting disease and heart affecting disease, haemorrhagic fever with renal syndrome (hfrs) sometimes called.
Puumala virus (puuv), hantavirus pulmonary syndrome with andes virus m genome segment-based dna analysis of andes virus and sin nombre virus. Puumala virus (puuv) is a hantavirus that causes a information from all three segments of the virus genome spatiotemporal dynamics of puumala hantavirus. Routine puumala virus (puuv) infection diagnosis is performed using serological commercial kits of which performances have not been established in real life, which use recombinant protein from strains from central or north europe molecular diagnostic of these infection is not the rule consequently.